Typhidot

Typhidot IgG & IgM (Dot EIA)

Typhidot is a revolutionary method of diagnosis. invented in Malaysia, it is first known qualitative antibody detection test design for rapid diagnosis of typhoid fever. Using the Dot EIA technique, presence of IgM and IgG antibodies against a specific antigen on the outer membrane of S. typhi are detected. A control clinical field trial among febrile children admitted to a hospital in an endemic area showed that Typhidot when compared to culture is sensitive (95%) and did not show any cross reactions. Despite being used in a study population with a high prior probability to typhoid, Typhidot showed a high negative predictive value of 96.1% and offers the advantages of speed (1 hour), simplicity and early diagnosis.

Benefits of test

Fast – results can be obtained in 1 hour
Convenient – diagnose typhoid fever with single serum sample
Simple – sample preparation not required
Results are easy to intepret
Detects both IgG & IgM – indicated the stages of infection
Minimum sample volume used
Rapid, sensitive and specific

Features

Platform : Enzyme immunoassay
Format : Dot blot strip
Detection : Human IgG and IgM against specific S. typhi
50kD OMP antigen
Specimen : Serum/ Plasma
Specimen volume : 5 Ul(3 hours method)
Shelf life : 12 months
Assay time : 1 or 3 hours

Performance Characteristics

Typhidot has been evaluated by in many endemic area including Malaysia, India, Indonesia, Pakistan and
Philippines. Most of the study reported sensitivity and specificity of more than 90%

Typhidot IgM Rapid Test

Typhidot Rapid IgM is an immunochromatography- based rapid test in cassette format. The test uses Salmonella typhi OMP antigen to detect IgM antibody to S. typhi in the sample within 10 minutes

Features

Platform : Immunochromatography
Format : Cassette
Detection : Human IgM against S. typhi OMP antigen
Specimen : Serum/ Plasma/ Whole blood
Specimen volume : 30Ul Serum/ 35ul Whole blood
Shelf life : 18 months
Assay time : 10 minutes

Benefits of test

Fast – results can be obtained in 10 minutes
Convenient – only a single serum sample is required
Simple to perform – only 3 steps and sample preparation not required
Results are easy to interpret – test line appears = positive
Equipment is not required
Minimum sample volume used

Performance Characteristics

In-house evaluation shows that Typhidot Rapid IgM has sensitivity and specificity above 85%.